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Glycogen BioAssay(TM) Kit

Cat no: G8169-25

Glycogen BioAssay(TM) Kit

Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in a-1,4 linkage, with branching via a-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. The Glycgogen bioassay kit is an easy and convenient assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (lambda-max = 570nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2mg/ml.\n\nKit Components:\nHydrolysis Buffer 1x25ml\nDevelopment Buffer 1x25ml\nOxiRed Probe lyophilized\nDMSO (anhydrous) 1x0.4ml\nHydrolysis Enzyme Mix 1x1vial\nDevelopment Enzyme Mix lyophilized\nGlycogen Standard (2.0mg/ml) 1x100ul.\n\nStorage and Stability:\nStore the kit at -20 degrees C, protect from light and moisture. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8169-25

Size

100Tests

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