Glycogen BioAssay(TM) Kit II (Colorimetric)

Supplier:

United States Biological

Cat no: 167843

Glycogen BioAssay(TM) Kit II (Colorimetric)

Glycogen serves as the main carbohydrate storage in animals and can be converted to glucose readily. It is primarily found in the liver and muscle tissues. Glycogen is a branched biopolymer comprising of alpha-1,4 linkage with alpha-1,6 linkages occurring every 8-10 glucose units along the backbone. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. \n\nSample Type:\nAnimal tissues: Liver, muscle, etc. Cell culture: Adherent or suspension cells.?\nIntended Use:\nThe assay can detect less than 4ug/ml of Glycogen in various tissues. Analysis of metabolism and cell signaling.\n\nTest Principle:\nThe Glycogen BioAssay(TM) kit II provides a simple, fast and robust way to measure Glycogen levels in various biological samples. This assay is suitable for measuring Glycogen levels in samples that contain reducing substances, which may interfere with the oxidase-based assays. In this assay, Glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces a colorless Probe to a colored product with strong absorbance at 450nm. \n\nKit Components:\nGlycogen Hydrolysis Buffer, 1x15ml\nGlycogen Development Buffer, 1x25ml\nHydrolysis Enzyme Mix, lyophilized, 1x vial\nDevelopment Enzyme Mix, lyophilized, 1x vial\nProbe, lyophilized, 1x vial\nGlycogen Standard (2mg/ml), 1x100ul\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167843

Size

100Tests

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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