Glycogen Phosphorylase Isoenzyme BB, Recombinant, Human (GPBB)

Glycogen Phosphorylase is an enzyme that catalyzes the degradation of Glycogen in animals by releasing glucose-1-phosphate from the terminal alpha-1,4-glycosidic bond. This enzyme exists in two forms: an active phosphorylated form (Phosphorylase A) and an inactive un-phosphorylated form (Phosphorylase B). Both a and b forms of phosphorylase exist as homodimers. In mammals, the major isozymes of glycogen phosphorylase are found in muscle, liver and brain tissue. Glycogen Phosphorylase- a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. Glycogen Phosphorylase can also be used as a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.\n\nRecombinant Human Glycogen Phosphorylase is a single, non-glycosylated, polypeptide chain having a molecular mass of 97kD.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nApplications:\nImmunoassays and Western Blot.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of Recombinant Glycogen Phosphorylase.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8170-05B

Size

5ug

Form

Supplied as a liquid in 40% glycerol.

Purity

(same/more than) 85% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Hormonally specific phosphorylation of cardiac troponin I and activation of glycogen phosphorylase. Brunton LL, Hayes JS, Nature 1979 Jul 5;280(5717):78-80 2. Identification, synthesis, and characterization of new glycogen phosphorylase inhibitors binding to the allosteric AMP site. Kristiansen M, Andersen B, Westergaard N, J Med Chem 2004 Jul 1;47(14):3537-45 3. Chicken ovalbumin upstream promoter-transcription factor I represses the transcriptional activity of the human muscle glycogen phosphorylase promoter in C2C12 cells. Ferrer-Martinez A, Marotta M, Haro D, Biochim Biophys Acta 2004 May 25;1678(2-3):157-62 4. Binding of the potential antitumour agent indirubin-5-sulphonate at the inhibitor site of rabbit muscle glycogen phosphorylase b. Comparison with ligand binding to pCDK2-cyclin A complex. Kosmopoulou MN, Leonidas DD, Bischler N, Oikonomakos NG, Eur J Biochem 2004 Jun;271(11):2280-90 5. Regulation of rat hepatocyte function by P2Y receptors: focus on control of glycogen phosphorylase and cyclic AMP by 2-methylthioadenosine 5'-diphosphate. Dixon CJ, Hall JF, Boarder MR, J Pharmacol Exp Ther 2004 Oct;311(1):334-41 6. Activation of glycogen phosphorylase with 5-aminoimidazole-4-carboxamide riboside (AICAR). Assessment of glycogen as a precursor of mannosyl residues in glycoconjugates. Shang J, Lehrman MA, J Biol Chem 2004 Mar 26;279(13):12076-80.