Glycogen Synthase Kinase 3 alpha, beta (GSK3a/b), phosphorylated (Tyr279/216)

Cat no: G8170-41A3

Supplier: United States Biological

0 reviews | Write a Review Pencil

Read More on Supplier Website

Description
Additional Information

Glycogen synthases kinase 3 alpha (GSK3-a) is a multifunctional Serine/Threonine protein kinase, one of two isoforms of GSK3 (1). Originally identified as a regulator of glycogen synthase, it has been shown to be involved in the hormonal control of several regulatory factors and transcription factors. GSK3-a activity is down regulated by phosphorylation on Serine 21 by PKB, or activated by phosphorylation on Tyrosine 279 (2). Insulin inactivates GSK3-a by rapid phosphorylation which leads to activation of Glycogen Synthase in the cells (3). GSK3-a activitiy has been linked to a pathogenic mechanism in Alzheimer's disease (4). GSK3 beta) plays a key role in insulin and Wnt signaling, phosphorylating downstream targets by default, and becoming inhibited following the extracellular signaling event (5). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000 Immunoprecipitation: 1:40 Immunohistochemistry: 1:100 Immunocytochemistry: 1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	G8170-41A3
Reactivities:	Human, Mouse, Rat
Hosts:	Rabbit
Applications:	Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	100ul
Form:	Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
P type:	Mab
Isotype:	IgG
Purity:	Supernatant
References:	1. Martinho RG, et al. EMBO J. 17:7239-7249, 1998. 2. Sanchez Y, et al. Science 277:1497-1501, 1997. 3. Lovestone S, et al. Curr Biol 4(12):1077-1086, 1994. 4. Dajani R, et al. Cell 105(6): 721-32.
Additional info:	Recognizes human GSKa phosphorylated on tyrosine 279 and human GSK3b phosphorylyated on tyrosine 216. Species Crossreactivity: mouse and rat

See Full Product Listings >

Glycogen Synthase Kinase 3 alpha, beta (GSK3a/b), phosphorylated (Tyr279/216)

Cat no: G8170-41A3

Get Quote

About Biosave

Product Categories

Information


Get a Quote Direct from United States Biological




By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*