Glycogen Synthase Kinase 3 alpha, phosphorylated (Ser21), Blocking Peptide (GSK3a)

This peptide is used to block Phospho-GSK-3a (Ser21) Rabbit mAb #G8170-40D5 reactivity.\n\nGlycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulated glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3a and Ser9 of GSK-3b (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).\n\nQuality Control:\nThe quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-GSK-3a (Ser21) Rabbit mAb #G8170-40D5 by immunohistochemistry.\n\nApplications:\nUse as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. Other applications not tested.\n\nDirections for Use:\nFor immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8170-40R

Size

100ug

Applications

IHC

Purity

Purified

References

1 Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279. 2 Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141. 3 Cross, D.A. et al. (1995) Nature 378, 785-789. 4 Nusse, R. (1997) Cell 89, 321-323. 5 Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.