Glycogen Synthase Kinase 3 beta (GSK3 beta, GSK-3 beta, GSK3b)

Glycogen synthase 3 alpha and beta (GSK-3alpha and GSK-3beta) are two highly related proteins of 51 and 47kD that function as broad specificity serine/threonine kinases (1). Although they are the products of distinct genes, GSK 3alpha and GSK3beta are 93% identical over the catalytic domain, with GSK3 alpha containing an additional 40 residues (2). The two isoforms are ubiquitously distributed, with the highest levels of expression occurring in the brain (2). GSK-3alpha is identical to multifunctional ATP-citrate lyase kinase and GSK3beta is identical to tau protein kinase 1 (TPK1) (1,3). GSK3beta is believed to be involved in the hyperphosphorylation of tau protein in the Alzheimer diseased brain, and in the regulation of binding of beta catenin to APC (4,5).\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:1,000\nImmunoprecipitation: 5-10ug\nImmunohistochemistry: Staining of Alzheimer neurons\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8170-44B

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat, Xen/Amph

Form

Supplied as a liquid in PBS, pH 7.4, 0.01% sodium azide.

P Type

Mab

Purity

Neat serum

Isotype

IgG2a

References

1. Hardie, G. and Hanks, S., Academic Press Limited, San Diego, CA; 418pps (1995). 2. Woodgett, J.R., EMBO J. 9: 2431-2438 (1990). 3. Ishiguro, K., et al., FEBS Lett. 325: 167-172 (1993). 4. Aplin, A.E., et al., NeuroReport 8: 639-643 (1997). 5. Rubinfeld, B., et al., Science 272: 1023-1026 (1996). 6. Ferrer, I., et al., Acta Neuropathol. 104(6): 583-591 (2002). 7. Knockaert, M., et al., J. Biol. Chem. 277(28): 25,493-25,501 (2002). 8. Ferrer, I., et al., Neuropathol. Appl. Neurobiol. 29(1): 23-34 (2003).

Additional Info

Recognizes GSK3 beta. Species crossreactivity: rat, human and Xenopus laevis.