Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3a and GSK-3b) share 85% amino acid identity. GSK-3b, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.
Hybridoma:
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived recombinant human Glycogen Synthase Kinase-3 beta (rhGSK3b; aa 1-420; Accession # P49841).
Recommended Dilution:
Western Blot: An antibody concentration of 1.0ug/ml is recommended. Optimal dilutions should be determined by the individual laboratory.
Optimal dilutions to be determined by the researcher.
Protocols for Immunoblotting:
Blotting Buffer Blocking Solution Antibody Solution 25 mM Tris, pH 7.4
0.15 M NaCl
0.1% Tween(R) 20 5% nonfat dry milk in Blotting Buffer Adjust pH to 7.4 5% nonfat dry milk in Blotting Buffer Adjust pH to 7.4
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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