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Glycogen Synthase Kinase 3 beta, phosphorylated (Ser9) (Glycogen Synthase Kinase-3 beta, GSK-3 beta, GSK3B)

Glycogen Synthase Kinase 3 beta, phosphorylated (Ser9) (Glycogen Synthase Kinase-3 beta, GSK-3 beta, GSK3B)

Cat no: G8170-41T

Supplier: United States Biological
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Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3a and Ser9 of GSK-3b (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:400 Flow Cytometry: 1:200 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G8170-41T
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
Alternative names: EC=2.7.11.26
References: (1) Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279. (2) Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 82, 135-141. (3) Cross, D.A. et al. (1995) Nature 378, 785-789. (4) Nusse, R. (1997) Cell 89, 321-323. (5) Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.
Additional info: Recognizes endogenous levels of GSK-3B, phosphorylated at (Ser9). Species Crossreactivity: hamster, mouse, rat.

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