Glycogen Synthase Kinase 3 beta, phosphorylated (Ser9) (GSK3b)

Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation. GSK 3B participates in the Wnt signaling pathway and has been implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. GSK 3B phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:50,000\nWestern Blot: 1:1500-1:3000.\nImmunohistochemistry: 1:200-1:1000\nImmunofluorescence: 1:200-1:1000.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nPositive Control:\nSerum starved 293T whole cell lysate.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8170-44H

Size

100ug

Applications

ELISA, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as liquid in PBS, pH7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Stambolic V. and Woodgett J.R. (1994) Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation. Biochem. J. 303: 701-704. 2. Rhoads A.R. et al. (1999) Radiation hybrid mapping of genes in the lithium-sensitive wnt signaling pathway. Mol. Psychiatry 4: 437-442. 3. Lau K.F. et al. (1999) Molecular cloning and characterization of the human glycogen synthase kinase-3beta promoter. Genomics 60: 121-128. 4. Delcommenne M. et al. (1998) Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase. Proc. Natl. Acad. Sci. U.S.A. 95: 11211-11216. 5. Hong Y.-R., et al (2000) Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta. Biochim. Biophys. Acta 1492: 513-516. 6. Dajani R. et al. (2001) Crystal structure of glycogen synthase kinase 3 beta: structural basis for phosphate-primed substrate specificity and autoinhibition. Cell 105: 721-732. 7. Bax B. et al. (2001) The structure of phosphorylated GSK-3beta complexed with a peptide, FRATtide, that inhibits betacatenin phosphorylation. Structure 9: 1143-1152.

Additional Info

Recognizes human phosphorylated human GSK3B, pS9. Assay by IEP resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated GSK3B.