Glycogen Synthase Kinase 3 beta, phosphorylated (Ser9) (GSK3b)

GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3a and GSK3b. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway. \n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000-20,000 \nImmunohistochemistry (paraffin): 1:100-250 \nImmunocytochemistry: 1:100-250\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control: \n293T cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8170-50C

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Plyte SE, et al. (1992). Biochim Biophys Acta. 1114(2-3):147-62. 2. Miller, J. R., et al. (1996). Genes Dev. 10: 2527-2539. 3. Boyle WJ, et al. (1991). T. Cell. 64(3):573-584. 4. Bellacosa, A., et al. (1992). P. N. Science 254: 244-247.

Additional Info

Recognizes human GSK3, beta phosphorylated on Ser9.