Glycogen Synthase Kinase 3 (GSK3)

Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. Two isoforms of GSK-3 are reported in mammals: a 51kD GSK-3a and a 47kD GSK-3b. These two isoforms exhibit about 98% homology in their kinase domains, but share only about 36% identity in the last 76 C-terminal amino acid residues. GSK-3a contains a glycine-rich extension at its N-terminus. A minor (~15% of total) splice variant of GSK-3b, GSK-3b2, has also been identified, which contains a 13-residue insert within the kinase domain. It exhibits reduced kinase activity towards tau protein compared with

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SPECIFICATIONS

Catalog Number

G8170-40

Size

100ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Dro/Arth

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

Hughes, K., et al., Eur. J. Biochem. 203: 305-311, 1992. Woodgett, J.R., et al., EMBO 12: 803-808, 1993. Siddle, K., et al., Biochem J. 305: 25-28, 1995. Application Harwood, et al.; Cell 80: 139-148, 1995.

Additional Info

Recognizes drosophila Glycogen Synthase Kinase 3 alpha (51kD) and beta (46kD) isoforms. Due to extremely high conservation among the known enzymes of many different species, should crossreact with nearly all species homologues. Species Crossreactivity: mouse, rat, S. pombe, Dictyostelium and human.