Glycogen synthase kinase 3, phosphorylated (Ser9)

Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3a and GSK3b. Specifically, GSK3b has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser9 phosphorylation underlies the inhibition of GSK3b by insulin (Sutherland et al., 1993).\n\nPositive Control: Rat cortex lysate\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8170-48

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Rat

Form

10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Sutherland C, Leighton IA, Cohen P (1993) Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. Nov 15;296 (Pt. 1):15-9.Papkoff J, Aikawa M (1998) WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. Jun 29;247(3):851-8.

Additional Info

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse, chicken, bovine, canine, non-human primate, Zebrafish and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Specific for the ~46k GSK3b protein phosphorylated at Ser9. Also weakly labels the ~51k GSK3a band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.