Glycogen Synthase, phosphorylated (Ser640)

Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.\n\nApplications: \nSuitable for use in Immunoblot and ELISA. Other applications not tested.\n\nRecommended Dilution(s): \nELISA: 1:30,000-1:150,000; Assayed against 0.1ug of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at RT. \nImmunoblot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8170-30A

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then\ncrossadsorbed against the non-phosphorylated form of the immunizing peptide.

Isotype

IgG

References

1. DiGiovanna, M.P., Stern, D.F and Roussel, Curr. Prot. Immunol. 11: 6.1-6.19 (1996).

Additional Info

Specific for the phosphorylated form of human muscle GS at S640. Minimally reactive (less than 1%) with the non-phosphorylated form of the immunizing peptide. Species Crossreactivity: Human and mouse.