Home  >  Products  >  Glycophorin A (Glycophorin-A, GPA, GYPA, CD235a, CD235a Antigen, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS, MN Sialoglycoprotein, PAS-2, Sialoglycoprotein alpha)
Glycophorin A (Glycophorin-A, GPA, GYPA, CD235a, CD235a Antigen, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS, MN Sialoglycoprotein, PAS-2, Sialoglycoprotein alpha)

Glycophorin A (Glycophorin-A, GPA, GYPA, CD235a, CD235a Antigen, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS, MN Sialoglycoprotein, PAS-2, Sialoglycoprotein alpha)

Cat no: G8177-14

Supplier: United States Biological
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GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. The antibody agglutinates untreated RBCs but it failes to agglutinate papain-treated cells. The antibody HIR2 significantly binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.1mg labels 10e6 human peripheral blood leukocytes. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G8177-14
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in PBS, 15mM sodium azide, pH7.4.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
Alternative names: Glycophorin A (MN Blood Group),
References: 1. Shen DC., Tang MH., Zhang JX. et al. (1993). A monoclonal antibody against glycophorin A. J. of Monoclonal Antibody. 9(2):59. 2. Nakahata T and Okumura N. Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401. 3. Rogers CE, Bradley MS, Palsson BO et al. Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597. 4. Bain BJ. Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990. 5. Keren DF, Hanson CA and Hurtubise PE, eds.. Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994. 6. Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London. 7. David M., A. Pascale, B. Armand et al. (2002). Leucocyte Typing VII: White Cell Differentiation Antigens. 577-582.
Additional info: Recognizes N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins.

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