GPV (platelet Glycoprotein V; also CD42d) is a 83kD member of the leucine-rich (LR) repeat family of proteins. On the platelet surface, it noncovalently interacts with three additional proteins to form a heteromeric complex that serves as a receptor for von Willebrand factor. Mature human GPV is a 544aa type I transmembrane glycoprotein. In its extracellular domain, it contains 15 LR repeats (aa73-472), and possesses a short 15aa cytoplasmic tail. GPV is naturally cleaved by ADAM10 and ADAM17 after Pro493, generating a 75-80kD soluble fragment.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant human GPV is ~3ng/well.
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse myeloma cells with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Glycoprotein V (aa17-253).
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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