Glycoprotein V (GP-V, CD42d, GP5, GPV)

GP-V (platelet glycoprotein V; also CD42d) is a 78-88kD member of the leucine-rich (LR) repeat family of proteins. It is expressed on the surface of platelets where it noncovalently interacts with three additional proteins to form a heteromeric complex that serves as a receptor for both thrombin and von Willebrand factor. In addition, GP-V is normally a receptor for collagen. Following receptor complex activation due to thrombin cleavage of GP-V, the collagen-binding activity of GP-V is lost, limiting thrombus growth at sites of clot formation. Mature mouse GP-V is a 551 amino acid (aa) type I transmembrane glycoprotein. In its 506 aa extracellular domain (aa17-522), GP-V contains 16 LR repeats (aa17-474), and terminates with a short 24aa cytoplasmic tail. Based on human, mouse GP-V might be naturally cleaved by thrombin and ADAM17, generating soluble fragments that range from 70-82kD in size. Over aa17-522, mouse GP-V shares 83% and 70% aa sequence identity with rat and human GP-V, respectively.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunocytochemistry: 5-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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