Glycoprotein V, Recombinant, Human (CD42d, GPV, Platelet glycoprotein V, GP5)

GPV (platelet glycoprotein V; designated CD42d) is an 83kD type I transmembrane (TM) glycoprotein of the leucine-rich repeat (LRR) family. It is expressed exclusively within the platelet/megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIba/b and GPIX, at a rati o of one GPV to two of each other subunit. The GPI-V-IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard-Soulier syndrome, a rare bleeding disorder. The human GPV cDNA encodes a 560aa protein with a 16aa signal sequence, a 507aa extracellular domain (ECD) containing 15 LRR, a 21aa TM sequence, and a short (16aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80kD by ADAM10 or ADAM17 cleavage after P507, or 69kD by thrombin cleavage after R476. High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin. The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expressi on of GPV requires GPIb. Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin.\n\nRecombinant protein corresponding to aa17-523 with a C-terminal 6-His tag from human Glycoprotein V expressed in NSO cells.\n\nMolecular Weight: 75-85kD under reducing conditions\n\nBiological Activity:\nMeasured by the ability of the immobilized protein to support the adhesion of thrombin-activated human platelets. When 5x10e6 cells/well are added to rhGPV coated plates (5ug/ml, 100ul/well),~50-70% will adhere after 1hr at 37 degrees C.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G4905

Size

50ug

Form

Supplied as a lyophilized powder from PBS. Reconstitute with 100ul sterile PBS.

Purity

(same/more than)95% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: (same/less than)1EU/ug (LAL)

References

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