Glyoxalase II (GLO2; also hydroxyacylglutathione hydrolase 2 and Glx II) is a monomeric, cytosolic 29kD member of the Glyoxylase II family, metallo-B-lactamase superfamily of enzymes. It is expressed in liver and kidney, and converts GLO1-generated lactoylglutathione into lactate and GSH. It is upregulated by p63 and p73, and thus serves as a p53-associated survival factor. Human GLO2 is 260aa in length. It binds two atoms of Zn and contains one substrate binding region (aa143-145 plus 249-252). The 29kD form represents 80-90% of cellular GLO2. There is also a 32-33kD, 308aa mitochondrial isoform that arises via the use of an alternative start site. Its function is unclear, given that mitochondria do not contain GLO1. There is an additional potential isoform that shows an insertion of nine aa after Thr167. Human GLO2 shares approximately 90% aa identity with mouse GLO2.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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