Glypican 5 (Glypiated Proteoglycan 5) (PE)

The glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that likely play a key role in embryonic morphogenesis. There are currently six known mammalian glypicans. They all share a common-sized protein core of 60-70kD, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of glypicans are known, with one subfamily containing glypicans-1, 2, 4 and 6, and another subfamily containing glypicans-3 and 5. 3, 5 Human glypican-5 (GPC-5) is synthesized as a 572aa preproprecursor that contains a 24aa signal sequence, a 532aa mature region and a 16aa C-terminal prosegment. \n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul to label 1x10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

G8235-56A

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Song, H.H. and J. Filmus, 2002,Biochim. Biophys. Acta 1573:241. 2. Filmus, J., 2001, Glycobiology 11:19R. 3. De Cat, B. and G. David, 2001, Semin. Cell Dev. Biol. 12:117. 4. Filmus, J. and S.B. Selleck, 2001, J. Clin. Invest.108:497. 5. Veugelers, M. et al., 1999, J. Biol. Chem. 274:26968. 6. Veugelers, M. et al., 1997, Genomics 40:24 7. Saunders, S. et al., 1997, Dev. Biol. 19:78. 8. Rapraeger, A. et al., 1985, J. Biol. Chem. 260:11046. 9. Fransson, L-A. et al., 2004, Cell Mol. Life Sci. 61:1016. 10. Fransson, L-A., 2003, Int. J. Biochem. Cell Biol. 35:125.

Additional Info

Recognizes human Glypican 5. Species Crossreactivity: mouse