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GM-CSF, Recombinant, Human, Animal Free (Granulocyte Macrophage Colony Stimulating Factor, AF, CSF-2. Pluripoietin-alpha, MGI1GM)

Cat no: 142883

GM-CSF, Recombinant, Human, Animal Free (Granulocyte Macrophage Colony Stimulating Factor, AF, CSF-2. Pluripoietin-alpha, MGI1GM)

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is hematopoietic factor produced by endothelial cells, monocytes, fibroblasts and T cells in response to a number of inflammatory mediators. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. Human and mouse GM-CSF show no cross-reactivity. \n\nSource:\nRecombinant protein corresponding to 128aa from human Granulocyte Macrophage Colony Stimulating Factor expressed in E.coli.\n\nMolecular Weight: \n~14.6kD\n\nEndotoxin:\n(same/less than)1EU/ug (LAL)\n\nBiological Activity:\nThe activity is determined by the dose-dependent induction of human TF-1 cell proliferation and is typically <0.1ng/ml.\n\nAA Sequence:\nMAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

142883

Size

5ug

Form

Supplied as a lyophilized powder in 10mM Na2PO4, pH 7.5. Reconstitute with sterile dH2O.

Purity

~95% (Reducing and non-reducing SDS-PAGE)

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