GM130

The Golgi apparatus functions in the modification, organization and transport of proteins and membranes targeted to other parts of the cell, such as the plasma membrane, lysosomes and endosomes. This regulated transport is important for appropriate protein localization, secretion and signal transduction. Members of the Golgin family of proteins, including GM130, Giantin, p115 and GRASP65, are defined by their presence in the Golgi matrix and by their long coiled coil domains. Golgin function, which is regulated in part by small GTPases of the Rab and Arl families, includes establishing and maintaining Golgi structure as well as transport (reviewed in 1). The Golgi cisternae are stacked and linked laterally to form a ribbon. GRASP65 and GM130 are required for membrane fusion events that mediate ribbon formation during Golgi assembly. These lateral fusion events allow for uniform distribution of Golgi enzymes (2). GM130 and Giantin interact with the transport factor p115 to facilitate endoplasmic reticulum (ER)-Golgi transport (3). GM130 is also involved in the transport of the Ether-a-go-go-Related (hERG) potassium ion channel, whose inappropriate localization may be an underlying cause in long Q-T syndrome, a hereditary and potentially fatal cardiac arrhythmia (4). Further, GM130 was implicated in signal transduction regulating invasion, migration and cell polarization via its interaction with and activation of serine/threonine kinases YSK1 and MST4 (5).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern blot 1:1000\nImmunoprecipitation 1:25\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\n\n

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