GM6001, Powder (Ilomastat, MMP Inhibitor, Galardin)

GM6001 MMP Inhibitor (Ilomastat) is a potent inhibitor of collagenase. Broad spectrum inhibitor of matrix metalloproteases (MMPs) with Ki factor in nM range. Widely used to inhibit MMPs in vitro experiments and in animal models.\n\nNomenclature: N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide\n\nReported Ki values are as follows (see Galardy Ref): \nHuman MMP-1 (Fibroblast collagenase): 0.4nM\nHuman MMP-3 (Stromelysin): 27nM\nHuman MMP-2 (72kD gelatinase): 0.5nM\nHuman MMP-8 (Neutrophil collagenase):0.1nM\nHuman MMP-9 (92kD gelatinase): 0.2nM\n\nApplications: \nSuitable for use in Cell Culture\n\nRecommended Dilutions:\nCell Culture: 10-25uM \nIt is recommended that the concentration of GM6001 in aqueous working solutions is determined upon dilution and confirmed prior to each use. Relative concentrations of GM6001 in aqueous solutions may be determined by measuring the absorbance of the solution (tyrosine) at 280nm.\n\nReconstitution: Reconstitute in DMSO (at least 25mM), aliquot and store at -20 degrees C. Other organic solvents (ethanol) have also been used. Further dilutions can be made with dH2O or with low salt buffers. G8237 will precipitate from aqueous solutions exceeding 100uM. Aqueous solution will hydrolyze or decompse at a rate of 1-2% per week. Sonication of the mixture will enhance dissolution.\n\nQuality Control:\nH-NMR Spectrum: Conforms\nMass Spectrum: 389 [M+1]\nThin Layer Chromatography (ethylacetate:methanol = 6:1): Single spot\n\nStorage and Stability:\nPowder is stable at RT for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8237

Size

1mg

Form

White to off-white powder

Purity

(same/more than)97% by HPLC

References

US Biological application reference: 1. Menchen. L. et al., (2009) Gut 58:920-928. 2. Phillips, D.D. et al., (2013) J. Biol. Chem. .M113.452110. 1. Galardy, R.E., et al., Ann. NY Acad. Sci. 732: 315-323 (1994). 2. Knight, C.G., et al., FEBS Letters 296: 263-266 (1992). 3. Levy, D.E., et al., Journal of Medical Chemistry 41: 199-223 (1998). 4. Turk, B.E., et al., Nature Structural and Molecular Biology 11(1): 60-65 (2004).

Alternative Names

N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide