GMR-alpha (CD116, Granulocyte-macrophage Colony-stimulating Factor Receptor Subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, CDw116, CSF2RA, CSF2R, CSF2RY)

CD116 is a 70-85kD a chain of the GM-CSF receptor. It combines with CDw131 B chain to form the high affinity GM-CSF receptor. A soluble form of CD116, which binds GM-CSF with a relatively low affinity, has been identified. In addition, an alternatively spliced form of CD116 with an altered cytoplasmic tail has been described. CD116 is expressed on various myeloid cells including monocytes, macrophages, neutrophils, eosinophils, dendritic cells and their precursors, fibroblasts, and endothelial cells. CD116 is expressed on myeloid leukemias, osteogenic sarcoma cell lines, osteoblast-like cells and breast and lung carcinoma cell lines.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: (same/less than)0.5ug/10e5 cells in 100ul\nImmunohistochemistry: acetone-fixed, frozen tissue sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8951-31G

Size

100ug

Applications

FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1,k

References

1. Stomski, F.C., et al. 1998. J. Biol. Chem. 273:1192. 2. McClure, B., et al. 2001. Blood 98:3165. 3. Guthridge, M,A., et al. 2004. Blood 103:820. 4. Miyajima, A., et al. 1993. Blood 82:1960.

Additional Info

Recognizes human CD116.