GNB2L1 (Guanine Nucleotide Binding Protein Subunit beta-2-like 1, Guanine Nucleotide-binding Protein Subunit beta-like Protein 12.3, Cell Proliferation-inducing Gene 21 Protein, Gnb2-rs1, H12.3, Human Lung Cancer Oncogene 7 Protein, HLC7, HLC-7, PIG21, Re

The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the b subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCbII and other classical PKC isoforms (1). RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways (reviewed in 2). One example of this is its enhancement of PKC-dependent JNK activation (3). RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well (reviewed in 4). RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation (5).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8250-37A

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Ron, D. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 839-843. 2.Lopez-Bergami , P. et al. (2005) Mol. Cell 19, 309-320. 3.McCahill, A. et al. (2002) Mol. Pharmacol. 62, 1261-1273. 4.Nilsson, J. et al. (2004) EMBO Rep. 5, 1137-1141. 5.Chang, B. Y. et al. (2002) Oncogene 21, 7619-7629.

Additional Info

Recognizes endogenous levels of total human GNB2L1. Species Crossreactivity: Mouse, rat and monkey.