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GnRH Receptor (LH-RH Receptor) (BSA & Azide Free)

GnRH Receptor (LH-RH Receptor) (BSA & Azide Free)

Cat no: G8256X

Supplier: United States Biological
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GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH). Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): 1:100 for 60 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Pituitary or normal ovary Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G8256X
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
Size: 100ul
Form: Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See G8256.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Karande AA, et al. 1995. Molecular and Cellular Endocrinology. 114:51-56.
Additional info: Recognizes human GnRH Receptor (LH-RH Receptor).

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