Syntabulin (also Golgi-localized synaphilin-related protein) is a 75kD molecule that belongs to the syntaxin-binding and kinesin-1 family of motor adaptor proteins. It is highly spliced, with various forms associated with different tissues. In neurons, it anchors syntaxin-1 to KIF5B, creating a complex by which synaptic components can be transported to synaptic terminals. Human Syntabulin is a type II transmembrane (TM) protein 663aa in length. It contains one coiled-coil region (aa271-353) and a TM domain between aa 606-626. Alternate splice forms show alternate start sites at Met120 and Met207, a five aa substitution for aa1-8, a 16aa
insertion after Lys8, and an eight aa substitution for aa1-76. Over aa486-653, human Syntabulin shares 83% aa identity with mouse Syntabulin.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.