Golgi Matrix Protein (GM130)

The Golgi apparatus functions in the modification, organization, and transport of proteins and membranes targeted to other parts of the cell, such as the plasma membrane, lysosomes, and endosomes. This regulated transport is important for appropriate protein localization, secretion, and signal transduction. Members of the Golgin family of proteins, including GM130, Giantin, p115, and GRASP65, are defined by their presence in the Golgi matrix and by their long coiled coil domains. Golgin function, which is regulated in part by small GTPases of the Rab and Arl families, includes establishing and maintaining Golgi structure and transport (reviewed in 1). The Golgi cisternae are stacked and linked laterally to form a ribbon. GRASP65 and GM130 are required for membrane fusion events that mediate ribbon formation during Golgi assembly. These lateral fusion events allow for uniform distribution of Golgi enzymes (2). GM130 and Giantin interact with the transport factor p115 to facilitate endoplasmic reticulum (ER)-Golgi transport (3). GM130 is also involved in the transport of the Ether-a-go-go-Related (hERG) potassium ion channel. Inappropriate hERG localization may be an underlying cause in Long QT syndrome, a hereditary and potentially fatal cardiac arrhythmia (4). Further, GM130 was implicated in signal transduction regulating invasion, migration, and cell polarization via its interaction with and activation of serine/threonine kinases YSK1 and Mst4 (5).\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence(Immunocytochemistry): 1:3200\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137439

Size

40ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG

References

1. Barr, F.A. and Short, B. (2003) Curr. Opin. Cell Biol. 15, 405-413. 2. Puthenveedu, M.A. et al. (2006) Nat. Cell Biol. 8, 238-248. 3. Alvarez, C. et al. (2001) J. Biol. Chem. 276, 2693-2700. 4. Roti, E.C. et al. (2002) J. Biol. Chem. 277, 47779-47785. 5. Preisinger, C. et al. (2004) J. Cell Biol. 164, 1009-1020.

Additional Info

Recognizes endogenous levels of total human GM130. May react with a protein of unknown origin at 30kD. Species Crossreactivity: monkey.