Golgi protein 58K (Formiminotransferase cyclodeaminase, FTCD, Glutamate formiminotransferase, LCHC1)

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.\n\nCellular Localization: Cytoplasmic and Golgi Apparatus\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:32,000\nWestern Blot: 0.03-0.1ug/ml. Predicted molecular weight: 59kD. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\n\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8577-50D

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Bloom GS & Brashear TA A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem 264:16083-92 (1989). <PUBMED:2777777>

Additional Info

Expected to recognize both reported isoforms. Species Crossreactivity: Reacts with Human. Predicted to react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Chicken (84% identity with immunogen), Zebrafish (90% identity with immunogen) and porcine (100% identity with immunogen) due to sequence homology.

Alternative Names

Formiminotransferase cyclodeaminase, FTCD, Glutamate formiminotransferase, LCHC1