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gp130 (IL-6 Family)

Cat no: G8580

gp130 (IL-6 Family)

GP130 is a signal-transducing subunit shared by the receptors for the IL-6 family of cytokines. The binding of a ligand to its receptor induces the dimerization of GP130, lending to activation of the Jak tyrosine kinase and tyrosine phosphorylation of GP130. These events lead to the activation of multiple signaltransduction pathways, such as the Stat, Ras-MAPK and PI3 kinase pathways whose activation is controlled by distinct regions of GP130.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2ug/ml detected gp130 in RIPA lysates from mouse 3T3/A31 fibroblasts. Previously detected gp130 in human A431 carcinoma cell lysate. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8580 (2ug/ml). Proteins were visualized using an IgG Gt xRb (HRP) and a chemiluminescence detection system. Gp130 migrated slightly faster than expected in the SDS-PAGE minigel system.\nImmunoprecipitation: 4ug immunoprecipitates gp130 from 500ug of RIPA lysates of mouse 3T3/A31 fibroblast and human A431 cell lysate.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nIncluded Positive Antigen Control: \nF4209: 3T3 cell lysate. Use 20ug of reduced lysate per lane for minigels. Add 2.5ul 2ME/100ul lysate and boil for 5 min to reduce the preparation.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8580

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Lin, S., et al., J. Clin. Invest.. 100: 1980-1990, 1997. 2. Weich, N., et al., Blood 90: 2893-3902, 1997.

Additional Info

Recognizes human gp130. Additional unknown proteins, Mr 32 and 50kD, detected in some cell lysates. Species Crossreactivity: mouse

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