Gp130/IL6ST BioAssay(TM) ELISA K, Human

Sandwich ELISA quantitative detection kit for Human Gp130/IL6ST in cell culture supernates, serum and plasma (heparin, EDTA, citrate).\n\nGP130(Glycoprotein 130) also known as пїЅIL6ST or CD130, пїЅis aпїЅtransmembrane proteinпїЅwhich is the founding member of the class of allпїЅcytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130пїЅshowed an apparent molecular mass of 130kD by SDS-PAGE. Gp130пїЅis involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-boundпїЅgp130. Gp130 is an important part of many different types of signaling complexes.Gp130пїЅmolecules with mutations in either of these segments could not transduce a growth signal.\n\nAssay Range:\n125pg/ml-8000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP40189\n\nEntrez Gene ID:\n3572\n\nKit Components:\n96-well plate pre-coated with anti-Gp130/IL6ST ELISA K, Human\nLyophilized recombinant Gp130/IL6ST ELISA K, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143856

Size

96Tests

References

1. Hibi, M., Murakami, M., Saito, M., Hirano, T., Taga, T., Kishimoto, T. Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell 63: 1149-1157, 1990.\n2. Narazaki, M., Yasukawa, K., Saito, T., Ohsugi, Y., Fukui, H., Koishihara, Y., Yancopoulos, G. D., Taga, T., Kishimoto, T. Soluble forms of the interleukin-6 signal-transducing receptor component gp130 in human serum possessing a potential to inhibit signals through membrane-anchored gp130. Blood 82: 1120-1126, 1993.\n3. Yoshida K, Taga T, Saito M, Suematsu S, Kumanogoh A, Tanaka T, Fujiwara H, Hirata M, Yamagami T, Nakahata T, Hirabayashi T, Yoneda Y, Tanaka K, Wang W-Z, Mori C, Shiota K, Yoshida N, Kishimoto T (1996).\n