GPR111, Recombinant, Human, aa19-375 (G-Protein-Coupled Receptor 111, PGR20)

GPR111, also known as PGR20, is an ~70kD member of the LN-7TM family of adhesion G protein-coupled receptors. Like other LN-7TM family proteins, it contains an extended N-terminal extracellular region followed by a series of seven transmembrane (TM) segments and a short C-terminal cytoplasmic tail (1, 2). The N-terminal domain contains a mucin-like stalk and a juxtamembrane region that resembles the GPS motif found in other LN-7TM proteins. The GPS motif is a component of the conserved GAIN domain which mediates the autoproteolysis and shedding of a wide range of proteins including LN-7TM proteins (3). The GPS-like motif in GPR111, however, is divergent from the consensus motif and does not appear to be cleavable (3, 4). Alternative splicing of human GPR111 generates a long isoform with a 92aa substitution for the N-terminal 24 residues as well as a 19aa substitution in the final TM segment and cytoplasmic tail. This recombinant protein product corresponds to the N-terminal extracellular domain of the shorter isoform (aa19-375). Within this region, human GPR111 shares ~68% aa sequence identity with mouse and rat GPR111. GPR111 is expressed in squamous epithelia of the skin, esophagus, tongue epidermis, and stomach (4).\n\nSource:\nRecombinant protein corresponding to aa19-375 from human GPR111, fused to 6-his tag at C-terminal, CHO-derived.\n\nMolecular Weight:\n~55-66kD\n\nEndotoxin:\n<0.10EU/1ug (LAL method)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute in sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145797

Size

50ug

Form

Supplied as a lyophilized powder in PBS. BSA free. Reconstitute in 500ug/ml PBS.

Purity

~95% (SDS

References

1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407. 2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23. 3. Arac, D. et al. (2012) EMBO J. 31:1364. 4. Promel, S. et al. (2012) Dev. Dyn. 241:1591.