GPR14 (UII Receptor)

Urotensins are neuropeptide secreted from the urophysis in the caudal neurosecretory system of the fishes. Two bioactive Urotensins, U1 and UII have been cloned from various species including mammals. UI is 41 aa peptide, which showed prolonged hypotensive effects in mammals. It is structurally similar to mammalian corticotropin-releasing factor. UII is a vasoactive 'somatostatin-like" 12-aa cyclic peptide with similarity to somatostatin-14. Human UII is composed of only 11 aa residues, while fish and frog UII possess 12 and 13 aa, respectively. The cyclic region of the UII, which is responsible for the biological activity of the peptide, has been fully conserved from fish to human. UII expression has been observed predominantly in the spinal cord. Recently, an orphan human G-protein coupled receptor homologous to rat GPR14 has been identified as the receptor for UII (2). GPR14 is expressed predominantly in cardiovascular tissue. Human UII effectively stimulated total peripheral resistance in anesthetized non-human primates, a response associated with profound cardiac contractile dysfunction. GPR14 (rat 386 aa and human 389 aa, ~75% homology) contains 7 transmembrane domains. It is most closely related to somatostatin receptor4. GPR14 is mostly expressed in the heart and pancreas, while low expression was detected in the brain.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well.\nWestern Blot: 1:1000- 1:5000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

G8584-99

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Coulouran Y et al (1999) FEBS Lett. 457, 28-32; Coulouran Y et al (1999) Proc. Natl. Acad. Sci. 95, 15803-15808 2. Ames RS et al (1999) Nature 401, 282-286; Marchese A et al (1995) Genomics 29, 335-344; Tal M et al (1995) BBRC 209, 752-759

Additional Info

Recognizes rat GPR14 (UII Receptor). Species sequence homology: mouse (100%), human (66%).