GPR35 initially identified as an orphan G-protein coupled receptor, was recently deorphanized using high-throughput intracellular calcium measurements in clonal cell lines expressing a chimeric G-protein alpha-subunit. Recent studies confirm kynurenic acid, a tryptophan metabolite as a ligand for GPR35. The protein shares homology with purinergic P2Y receptors, lysophosphatidic acid receptor GPR23, nicotinic acid receptor HM74, and the cannabinoid receptor GPR55. Expression analysis revealed prominent expression of the protein in immune and gastrointestinal tissues, suggesting potential physiological roles for GPR35 in these organs. The potential role of the protein involves regulating neuronal excitability and synaptic release. The protein has also been reported as a potential oncogene implicated in gastric cancer. The gene encoding for human GPR35 is localized in the chromosomal region 2q37.3.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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