Control of appetite involves a number of gastrointestinal hormones that belong to the ghrelin-motilin ligand family that either stimulate food intake and gastric emptying or inhibit these responses. These hormones have been the focus of intense research in hopes of developing drug therapy for gastrointestinal and eating disorders.
GPR39 is a G protein-coupled receptor (GPCR) isolated from human genomic libraries. It is sequentially distantly related to the growth hormone secretagogue receptor (ghrelin) and neurotensin receptors. Its endogenous ligand was unknown until 2005, when an amidated 23aa peptide, obestatin, was found to bind to it specifically. Obestatin was found through bioinformatics studies as a peptide, part of the ghrelin precursor. Interestingly, these two peptides appear to have opposite effects. While ghrelin stimulates appetite by acting on its receptor in the arcuate nucleus, obestatin suppresses appetite.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
ELISA: 1:100,000 using 50-100ng control peptide G8600-43G per well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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