Glutathione Peroxidase 1 (GPX1), also known as Cellular Glutathione Peroxidase, is a 201aa, 23kD member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX1 is a 92kD homotetramer consisting of four identical 23kD subunits, each containing a selenocysteine residue at the active site. GPX1 is an important antioxidant enzyme and protects red blood cells and other cells from oxidative damage. Deficiencies in red cell GPX1 have been associated with hemolytic anemia. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:32,000
Western Blot: 1-3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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