Gr-1 (GR1, Myeloid Differentiation Antigen, Ly-6G) (Azide Free)

Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein.1 In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. In the periphery, Ly-6G is expressed predominantly on neutrophils.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot, In vitro activation of complement-dependent Cytoxicity Assays and Depletion of Ly-6G+ cells. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G8750-02F

Size

500ug

Applications

FC, IHC, IP, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS. No preservative added.

P Type

Mab

Purity

Purified

Isotype

IgG2b,k

References

1. Fleming, T.J., M.L. Fleming and T. R. Malek. 1993. J. Immunol. 151:2399. 2. Hestdal, K., F.W. Ruscetti, J.N. Ihle, et al. 1991. J. Immunol. 147:22. 3. Lewinsohn, D.M., R.F. Bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313. 4. Lagasse, E., and I.L. Weissman. 1996. J. Immunol. Meth. 197:139. 5. Southern Biotechnology Associates, Inc. Unpublished observations. 6. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, et al. 1988. Eur. J. Immunol. 18:1819. 7. Brummer, E., A.M. Sugar, and D.A. Stevens. 1984. J. Leukocyte Biol. 36:505. 8. Stoppacciaro, A., C. Melani, M. Parenza, et al. 1993. J. Exp. Med. 178:151. 9. Tumpey, T.M., S.-H. Chen, J.E. Oakes, and R.N. Lausch. 1996. J. Virol. 70:898. 10. Conlan, J.W., and.J. North. 1994. J. Exp. Med. 179:259. 11. Czuprynski, C.J., J.F. Brown, N. Maroushek, R.D. Wagner, and H. Steinberg. 1994. J. Immunol. 152:1836. 12. Rakhmilevich, A.L. 1995. J. Leukoc. Biol. 57:827.

Additional Info

Recognizes mouse Ly-6G, Mr 21-25kD.