Home  >  Products  >  Gr-1 (GR1, Myeloid Differentiation Antigen, Lymphocyte Antigen 6 Complex Locus G, Lymphocyte Antigen 6G, Ly6g, Ly-6G, Ly-6G.1)
Gr-1 (GR1, Myeloid Differentiation Antigen, Lymphocyte Antigen 6 Complex Locus G, Lymphocyte Antigen 6G, Ly6g, Ly-6G, Ly-6G.1)

Gr-1 (GR1, Myeloid Differentiation Antigen, Lymphocyte Antigen 6 Complex Locus G, Lymphocyte Antigen 6G, Ly6g, Ly-6G, Ly-6G.1)

Cat no: G8750-01A

Supplier: United States Biological
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Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein.1 In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow.2 In the periphery, Ly-6G is expressed predominantly on neutrophils.3, 4 Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), Immunoprecipitation, and Westen Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8750-01A
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 250ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
References: 1. Fleming, T.J., M.L. Fleming and T. R. Malek. 1993. J. Immunol. 151:2399. 2. Hestdal, K., F.W. Ruscetti, J.N. Ihle, et al. 1991. J. Immunol. 147:22. 3. Lewinsohn, D.M., R.F. Bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313. 4. Lagasse, E., and I.L. Weissman. 1996. J. Immunol. Meth. 197:139. 5. Southern Biotechnology Associates, Inc. Unpublished observations. 6. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, et al. 1988. Eur. J. Immunol. 18:1819. 7. Brummer, E., A.M. Sugar, and D.A. Stevens. 1984. J. Leukocyte Biol. 36:505. 8. Stoppacciaro, A., C. Melani, M. Parenza, et al. 1993. J. Exp. Med. 178:151. 9. Tumpey, T.M., S.-H. Chen, J.E. Oakes, and R.N. Lausch. 1996. J. Virol. 70:898. 10. Conlan, J.W., and.J. North. 1994. J. Exp. Med. 179:259. 11. Czuprynski, C.J., J.F. Brown, N. Maroushek, R.D. Wagner, and H. Steinberg. 1994. J. Immunol. 152:1836. 12. Rakhmilevich, A.L. 1995. J. Leukoc. Biol. 57:827.
Additional info: Recognizes mouse Gr-1 antigen.

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