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Granulocyte Colony Stimulating Factor, 127aa, Recombinant, Human (G-CSF, Filgrastim, Lenograstim, Pluripoietin)

Cat no: G8950-34A


Supplier: United States Biological
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Recombinant GM-CSF comprises a 127 amino acid fragment (18-144) corresponding to the mature GM-CSF protein and is expressed in E. coli with an amino-terminal hexahistidine tag. GM-CSF is a potent species-specific growth factor produced by a variety of cell types including T cells, B cells, macrophages, mast cells and endothelial cells. GM-CSF is produced in response to cytokine or immune stimulation and has been shown to stimulate the proliferation, maturation and function of hematopoietic cells. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8950-34A
Applications: ELISA
Size: 10ug
Purity: Purified. (same/more than) 95% by SDS-PAGE.
References: Burgess, A.W. et al. (1987) Blood 69 (1) : 43-51 Metcalf, D et al. (1986) Blood 67 (1) : 37-45.

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