Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) is a glycoprotein produced mainly by monocytes as well as bone marrow stromal cells, fibroblasts, macrophages, endothelial cells. G-CSF induces the survival, proliferation, differentiation and activation of neutrophilic granulocyte precursor cells and it activates mature blood neutrophils. G-CSF is the most potent of the colony-stimulating factors to stimulate terminal differentiation into granulocytes and macrophages in leukemic myeloid cell lines. G-CSF plays an important role in defense against infection, in inflammation and in the maintenance of steady state hematopoiesis. G-CSF is a mitogen for some human myeloid leukemia cells and also for some carcinoma cell lines. G-CSF synergizes with some other cytokines, including GM-CSF and IL-4.
Applications:
Suitable for use in ELISA and Western Blot.
Other applications have not been determined.
Recommended Dilutions:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 6 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 1 month at 4 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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