Home  >  Products  >  Granulocyte Colony Stimulating Factor (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin) (Biotin)
Granulocyte Colony Stimulating Factor (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin) (Biotin)

Granulocyte Colony Stimulating Factor (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin) (Biotin)

Cat no: G8950-03E1

Supplier: United States Biological
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Granulocyte colony stimulating factor (G-CSF) is a 19.6kD haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which stimulates the development of bone marrow progenitor cells and in particular those committed to neutrophil lineage. The ability of G-CSF to stimulate the proliferation/differentiation and function of neutrophil granulocyte progenitor cells and survival of mature neutrophils, has resulted in G-CSF becoming an important factor in patient treatment following allogeneic (bone marrow/stem cell) transplantation and chemotherapy. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.25-1ug/ml. May be used in either direct ELISA or as a detection antibody in sandwich ELISA for human G-CSF in combination with G8950-03E as a capture reagent. Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8950-03E1
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Western Blot
Conjugates: Biotin
Size: 50ug
Form: Supplied as a lyophilized powder from PBS. No preservatives added. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Hill, C.P. et al. (1993) The structure of granulocyte colony-stimulating factor and its relationship to other growth factors. P.N.A.S. 90: 5167-5171. 2. Morton, J. et al. (2001) Granulocyte-colony-stimulating factor (G-CSF)-primed allogeneic bone marrow: significantly less graft-versus-host disease and comparable engraftment to G-CSF-mobilized peripheral blood stem cells. Blood. 98: 3186-3191.
Additional info: Recognizes human Granulocyte Colony Stimulating Factor.

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