Granulocyte Colony Stimulating Factor (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin) (Biotin)

Granulocyte colony stimulating factor (G-CSF) is a 19.6kD haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which stimulates the development of bone marrow progenitor cells and in particular those committed to neutrophil lineage. The ability of G-CSF to stimulate the proliferation/differentiation and function of neutrophil granulocyte progenitor cells and survival of mature neutrophils, has resulted in G-CSF becoming an important factor in patient treatment following allogeneic (bone marrow/stem cell) transplantation and chemotherapy.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nELISA: 0.25-1ug/ml. May be used in either direct ELISA or as a detection antibody in sandwich ELISA for human G-CSF in combination with G8950-03E as a capture reagent.\nWestern Blot: 0.1-0.2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8950-03E1

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS. No preservatives added. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hill, C.P. et al. (1993) The structure of granulocyte colony-stimulating factor and its relationship to other growth factors. P.N.A.S. 90: 5167-5171. 2. Morton, J. et al. (2001) Granulocyte-colony-stimulating factor (G-CSF)-primed allogeneic bone marrow: significantly less graft-versus-host disease and comparable engraftment to G-CSF-mobilized peripheral blood stem cells. Blood. 98: 3186-3191.

Additional Info

Recognizes human Granulocyte Colony Stimulating Factor.