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Granulocyte Colony Stimulating Factor (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin)

Granulocyte Colony Stimulating Factor (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin)

Cat no: G8950-03E

Supplier: United States Biological
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The ability of G-CSF to stimulate the proliferation/differentiation and function of neutrophil granulocyte progenitor cells and survival of mature neutrophils, has resulted in G-CSF becoming an important factor in patient treatment following allogeneic (bone marrow/stem cell) transplantation and chemotherapy. To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 ug/ml of this antibody is required. Applications: Suitable for use in ELISA, Functional Assays, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 0.25ug/ml ELISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 0.4-0.6ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human angiosarcoma Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8950-03E
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile 40-50% glycerol, ddH2O.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Hill, C.P. et al. (1993) The structure of granulocyte colony-stimulating factor and its relationship to other growth factors. P.N.A.S. 90: 5167-5171. 2. Morton, J. et al. (2001) Granulocyte-colony-stimulating factor (G-CSF)-primed allogeneic bone marrow: significantly less graft-versus-host disease and comparable engraftment to G-CSF-mobilized peripheral blood stem cells. Blood. 98: 3186-3191.
Additional info: Recognizes human G-CSF.

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