Granulocyte Colony Stimulating Factor Receptor (G-CSFR, CD114)

Granulocyte colony stimulating factor (GCSF) is a pleiotropic cytokine best known for its specific\neffects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic and granulocyte lineage (1). GCSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Cell activation by GCSF is mediated by granulocyte colony stimulating factor receptor alpha (GCSF\nR? also CD114), a 95 105kD type I transmembrane protein and member of the cytokine receptor superfamily, type I cytokine receptor family, and type 2 subfamily of receptor proteins. Mouse GCSF R is synthesized as an 837 amino acid (aa) precursor that contains a 25 aa signal sequence, a 601 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 187 aa cytoplasmic tail. The ECD contains one Ig like C2type domain, five fibronectin typeIII\ndomains, and 11 potential sites for N-linked glycosylation. Within the ECD there is also a WSXWS motif (aa 319 323) that is necessary for proper protein folding and thereby efficient intracellular transport and cellsurface receptor binding (2). Also, within the cytoplasmic domain there is a Box 1 motif which is required for JAK interaction and/or activation (1). Mouse GCSF R shares 63% aa sequence identity with human GCSF R. GCSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta (1). Mutations have been found in the gene encoding GCSF R in some patients with severe congenital neutropenia (1). These mutations typically lead to a truncation in the cytoplasmic domain of the G CSF R leading to\nmaturation arrest of neutrophilic precursors in the bone marrow and neutropenia in peripheral blood (3). Binding of GCSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases (2). Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3 and STATG), srcrelated protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon GCSF stimulation (4).\n\nSource: \nRecombinant corresponding to Met1-Asp626 from mouse GCSF R/CD114, C-terminal, 6-His tag expressed in NSO cells. \n\nMolecular Weight: \n~68.2kD\n\nBiological Activity:\nMeasured by its ability to inhibit the G-CSF-induced proliferation of NFS-60 mouse myeloid cells. The ED50 for this effect is typically 0.02-0.12ug/ml in the presence of 0.125ng/mL of recombinant mouse G-CSF.\n\nEndotoxin Level:\n<1EU/ug (LAL method)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8950-50N

Size

50ug

Form

Supplied as a lyophilized powder from PBS, Trehalose. Reconstitute with streile PBS to a concentration of 0.1mg/ml.

Purity

~95% (SDS-PAGE)

References

1. Ward, A.C. (2007) Front. Biosci. 12:608. 2. Layton, J.E. and N.E. Hall (2006) Front. Biosci. 11:3181.\n3. Mitsui, T. et al. (2003) Blood 101:2990. 4. Nicola, N.A. in Cytokine Reference, 2001, Oppenheim, J.J.\nand M. Feldmann, eds. Academic Press p.1935.