Granulocyte Colony Stimulating Factor, Recombinant, Human (CSF3, Colony Stimulating Factor 3 (Granulocyte), Filgrastim, GCSF, G-CSF, Granulocyte Colony-stimulating Factor Precursor, Lenograstim, MGC45931, Pluripoietin)

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-a and IFN-g. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-a activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.\nIn humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.\n\nBiological Activity: \nFully biologically active when compared to standard. The ED50 as calculated by the dose-dependant proliferation of murine NFS-60 is < 0.1ng/ml, corresponding to a Specific Activity of 1x10e7 IU/mg.\n\nAA Sequence:\nTPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or or an aqueous buffer containing 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8951-26P

Size

2ug

Reactivities

Hum

Form

Supplied as a lyophillized powder from 10mM sodium acetate buffer, 5% trehalose, pH 4.0. Reconstitute in sterile ddH2O or an aqueous buffer containing 0.1% BSA to 0.1-1.0mg/ml.

Purity

~98% by SDS-PAGE and HPLC analyses.