Granulocyte Colony Stimulating Factor, Recombinant, Mouse (G-CSF)

Granulocyte Colony Stimulating Factor (G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Murine G-CSF is a 20kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins , TNF , Interleukin-1 and GM-CSF . Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.\n\nRecombinant Murine G-CSF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 179 amino acids and having a molecular mass of 19kD. \n\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 0.51 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of G-CSF as a Reference Standard. \n\nDimers and aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel. \n\nBiological Activity:\nG-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is (same/less than) 0.05ng/ml, corresponding to a Specific Activity of 2x10e7 IU/mg. \n\nAA

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G8950-16B

Size

2ug

Form

Supplied as a lyophilized powder. No stabilizing proteins added. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of (same/more than)100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than) 95% by RP-HPLC, Anion-exchange FPLC, reducing and non-reducing SDS-PAGE silver stained gel. Endotoxin: (same/less than) 0.1ng/ug (1EU/ug)

References

1. Subcutaneous administration of granulocyte colony stimulating factor and stem cell factor ameliorates the outcome of acute myocardial infarction. Chin Med J (Engl) 2005 Aug 5;118(15):1303-7\n2. [Cyclic neutropenia with anti-NA2 antibodies and treatment with recombinant granulocyte colony-stimulating factor] An Pediatr (Barc) 2005 Aug;63(2):180-2\n3. [A case of granulocyte-colony stimulating factor producing small cell carcinoma of esophagus] Nippon Shokakibyo Gakkai Zasshi 2005 Jul;102(7):888-93\n4. CHOP versus CHOP plus granulocyte colony-stimulating factor in elderly patients with aggressive non-Hodgkin's lymphoma. J Clin Oncol 2005 Jul 20;23(21):4797-9; author reply 4799-800\n5. Primary serous carcinoma of the peritoneum producing granulocyte colony-stimulating factor. Acta Obstet Gynecol Scand 2005 Aug;84(8):820-2\n6. Granulocyte colony-stimulating factor (G-CSF) could enhance Fcgamma receptor expression in neutrophils of patients with B-cell lymphoma treated with rituximab. Leuk Lymphoma 2005 May;46(5):789-91\n