Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl

GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors \n\nRecombinant Human GM-CSF is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14,477D..\n \nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically. \n\nDimers and Aggregates:\n(same/less than)1% as determined by silver-stained SDS-PAGE gel analysis. \n\nEndotoxin: (same/less than) 0.1ng/ug (IEU/ug)\n\nBiological Activity:\nGM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.1ng/ml, corresponding to a Specific Activity of ~11x10e6IU/mg. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding with sterile dH2O, 0.1% HSA or BSA, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8950-10A

Size

2ug

Form

Supplied as a lyophilized powder from 2mM sodium phosphate buffer, pH 7.4. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.\n

Purity

(same/more than) 98% by RP-HPLC and anion-exchange FPLC, and reducing/non-reducing SDS-PAGE (silver stained).

References

1. Alenzi FQ, Br J Biomed Sci 2004;61(4):200-5 2. Kamata N, Kutsuna H, Kato T, Int J Hematol 2004 Dec;80(5):421-7 3. Chan RJ, Leedy MB, Voorhorst CS, Blood 2005 Jan 11; 4. Westcott MM, Abi-Habib RJ, Willingham MC, Frankel AE, Mol Cancer Ther 2004 Dec;3(12):1681-9 5. Ren H, Lu D, Zhonghua Xue Ye Xue Za Zhi 1997 Mar;18(3):137-9 6. Marshall JL, Gulley JL, Beetham PK, Doren S, J Clin Oncol 2004 Dec 21.