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Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl

Cat no: G8951-26Q

Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl

Recombinant Granulocyte Macrophage CSF is a single, non-glycosylated, polypeptide chain containing 127aa. The sequence of the first five N-terminal amino acids was determined to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically.\n\nSource: E. coli\n\nMolecular Weight: ~14.4kD\n\nActivity: \nThe ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is <0.1ng/ml, corresponding to a specific activity of 11.1x10e6 IU/mg. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for at least 6 months at -20 degrees C. Reconstitute with sterile deionized water to a concentration (same/more than)100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8951-26Q

Size

10ug

Applications

ELISA

Form

Supplied as a lyophilized powder from 2mM sodium phosphate, pH 7.4. No preservative added. Reconstitute with sterile deionized water to a concentration (same/more than)100ug/ml.

Purity

(same/more than)98% (RP-HPLC and SDS-PAGE). Purified by chromatographic techniques.

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