Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, GMCSF, Colony-stimulating Factor, CSF, Colony Stimulating Factor 2 (Granulocyte-macrophage), CSF2, MGC131935, MGC138897, Molgramostin, Sargramostim)

Source:\nRecombinant corresponding to 127aa of human Granulocyte Macrophage-Colony Stimulating Factor expressed in E.coli is a single, non-glycosylated, polypeptide chain.\n\nMolecular Weight:\n~14.5kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.1ng/ml, corresponding to a Specific Activity of 11.1x10 6IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 0.963 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8951-26T

Size

10ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Adenosine potentiates stimulatory effects on granulocyte-macrophage hematopoietic progenitor cells in vitro of interleukin-3 and stem cell factor, but not those of granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor and interleukin-11. Physiol Res 2005 Dec 12; 2. Acute CD4+ T lymphocyte-dependent interleukin-1-driven arthritis selectively requires interleukin-2 and interleukin-4, joint macrophages, granulocyte-macrophage colony-stimulating factor, interleukin-6, and leukemia inhibitory factor. Arthritis Rheum 2005 Dec;52(12):3749-3754. 3. Granulocyte-macrophage colony-stimulating factor as an arteriogenic factor in the treatment of ischaemic stroke. Expert Opin Biol Ther 2005 Dec;5(12):1547-56. 4. [The pulmonary expression of granulocyte-macrophage colony-stimulating factor and surfactant protein in adult idiopathic pulmonary alveolar proteinosis.]. Zhonghua Nei Ke Za Zhi 2005 Nov;44(11):832-5. 5. A vesicular stomatitis virus recombinant expressing granulocyte-macrophage colony-stimulating factor induces enhanced T-cell responses and is highly attenuated for replication in animals. J Virol 2005 Dec;79(24):15043-53. 6. Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts: a placebo controlled randomized study in healthy subjects. Eur J Med Res 2005 Oct 18;10(10):411-8.

Alternative Names

Granulocyte-macrophage Colony-stimulating Factor