Granulocyte Macrophage-Colony Stimulating Factor, Recombinant, Rat (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Plur

The overall structure of GM-CSF is highly compact and globular with a predominantly hydrophobic core, is characterized by a 4-alpha-helix bundle. The helices are arranged in a left-handed anti-parallel fashion, with two overhand connections. Within the connections is a two-stranded anti-parallel beta-sheet. The tertiary structure has a topology similar to that of (pig) growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus . \nGranulocyte Macrophage Colony Stimulating Factoris produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. \nGM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL-2 beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors. \nRecombinant Rat GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton. \nRat GM-CSF is purified by proprietary chromatographic techniques. \n\nAmino acid sequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg. \n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of the proliferation of murine MC/9 cells is 0.001-0.01ng/ml.\n\nProtein content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 0.966 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of Rat GM-CSF as a Reference Standard. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8950-23

Size

2ug

Form

Supplied as a lyophilized powder without additives. Reconstitute in sterile ddH20, 0.1% BSA or HSA to (same/more than)100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than) 95% by RP-HPLC or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.