Granzyme B (C11, Cathespin G Like 1, CCP1, CGL1, CSPB, CTLA1,CTSGL1, Cytotoxic serine protease B, Cytotoxic T Lymphocyte Cytotoxic T lymphocyte proteinase 2, EC 3.4.21.79, Fragmentin 2, Granzyme 2, GRB, GZMB, HLP, Human lymphocyte protein, Lymphocyte prot

Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes A to G, have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified. One of these, human granzyme B, is involved in target cell apoptosis during lymphocyte mediated cytotoxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell leads to the induction of DNA fragmentation and apoptosis.\n\nApplication(s): Flow Cytometry\nImmunocytochemistry\nImmunohistochemistry (frozen sections, paraffin sections)\nImmunoprecipitation\nWestern Blot\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8957-15J

Size

100ug

Applications

FC, ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Liquid. Purified antibody in PBS containing 0.02% sodium azide.\n

P Type

Mab

Purity

95% (SDS-PAGE).

Isotype

IgG1

References

Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation: S. Legros-Maida, et al.; Eur. J. Immunol. 24, 229 (1994) Abstract\nPerforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients: M.V. Clement, et al.; Transplantation 57, 322 (1994) Abstract\nGranzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor: C. Berthou, et al.; Blood 86, 3500 (1995) Abstract\nExpansion of a peripheral blood perforin+ CD8+ T-cell subset in long- term surviving lung transplanted patients: C. Berthou, et al.; Transplant. Proc. 28, 1964 (1996) Abstract\nAcquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense: C. Berthou, et al.; J. Immunol. 159, 5293 (1997) Abstract

Additional Info

Recognizes human granzyme B.