Granzyme B is a serine protease found in the lymphocyte granules of activatedcytotoxic T cells (CTL) and natural killer (NK) cells. It is one of several homologous serine proteinases called granzymes. The expression of the protein is correlated with the cytolytic potential of cytotoxic lymphocytes.The protein is critically involved in the rapid apoptotic signal delivered by CTL and NK cells. Following adhesion of the cytotoxic cell to the target, it is directly secreted and enters the target cell, assisted by perforin. Recently, the discovery of a receptor for granzyme B has indicated that granzymes might be taken up by receptor-mediated endocytosis and that perforin acts to release granzymes that are sequestered in endosomes into the cytosol of the target cell. It is necessary for target cell lysis in cell-mediated immune responses. The enzymatic activity of granzyme B is considered essential to its ability to induce cell death through the activation of caspases (caspase 3, 7, 9 and 10), but the exact mechanism remains unclear. The gene is composed of five exons and four introns and maps to 14q11.2 region of human chromosome.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:100-250
Immunohistochemistry (formalin fixed paraffin embedded): 1:10-1:25
Optimal dilutions to be determined by the researcher.
Positive Control:
Human T cell line
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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